Eppendorf-New Brunswick - FibraStage™(500ml)


應用:

FibraStageTM是一種拋棄式細胞培養系統,搭配FibraCel Disk使用,可提供低剪切力,高通氣量,無泡沫及大量細胞貼附表面積的細胞培養環境,以簡化傳統複雜的細胞培養方式且增加細胞及其它產物產率.可廣泛應用於人類蛋白質體學,基因治療,新藥研發,細胞治療及組織工程,培養細胞,製造大量重組蛋白質,單株抗體,病毒,疫苗及細胞組成物.

 

特性:

■ FibraStageTM培養系統包含已滅菌之500ml細胞培養瓶,內含有10克FibraCel Disk及可調整升降速度培養控制台

■ 產物產率等同於數個spinners,12個rollerbottles及數百個T-flasks

■ 對於懸浮性或貼附性細胞培養,密度可高達2.4x1010 cells

■ 使用者可簡易更換培養基質或產物回收

■ 可依需求改變操作台上升或下降速率及停留時間,用以控制細胞暴露於培養基或空氣中時間

■ 無須蒸氣,水或高溫滅菌釜等週邊設備,只需將FibraStageTM 系統置入二氧化碳培養箱,可直接作細胞培養

Introducing FibraStage, a disposable cell culture system that produces high product yields while decreasing labor demands over traditional cell culture devices.

FibraStage is an inexpensive and easy-to-use system, capable of producing mg quantities of protein, virus or biomass from a wide variety of anchorage-dependent or suspension cultures. Depending on your cell line and desired end product, up to four FibraStage bottles can produce yields of 2.4 x 10 10 with minimal effort. A single FibraStage bottle can produce a high-cell density of 6 x 10 9. Here's how:

Disposable FibraStage bottles are pre-sterilized and pre-loaded with 10 grams of FibraCel disks and each bottle can hold up to 500 mL of liquid. FibraCel disks are a unique solid-support matrix for producing high-density cultures. Cells grow in, on and between the porous, multi-layer disks, which provide an extremely high surface area for cell growth, equivalent to 12,000 cm2. To obtain an equivalent surface area of a single FibraStage bottle, one would need to maintain numerous spinners, dozens of roller bottles and hundreds of T-flasks.

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