The NucleoCassette is a highly specialised device, which holds the key to several of the unique features of NucleoCounter. The cassette is pre-loaded with a dye and its construction is opotimised for the mixing of sample and dye. It contains a measurement chamber where the fluorescent image is recorded. |
Approximately 50 ul of the sample mixture is loaded into the NucleoCassette. Within the NucleoCassette a nuclei staining dye has been immobilised (Propidium iodide). The sample dissolves the dye and after mixing the mixture reaches the transparent measurement chamber where the fluorescent image is recorded. |